Identification of Acrosome-reacted Goat Spermatozoa by a Trypan Blue-Giemsa Method
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چکیده
منابع مشابه
Targeted and restricted complement activation on acrosome-reacted spermatozoa.
A specific hypoglycosylated isoform of the complement regulator membrane cofactor protein (MCP; CD46) is expressed on the inner acrosomal membrane (IAM) of spermatozoa. This membrane is exposed after the acrosome reaction, an exocytosis event that occurs upon contact with the zona pellucida. We initiated this investigation to assess MCP's regulatory function in situ on spermatozoa. Upon exposur...
متن کاملFluorometric Viability Assessment of Capacitated and Acrosome-Reacted Boar Spermatozoa by Flow Cytometry
Sperm capacitation involves functional changes, such as the removal or appearance of specific molecules and changes in the plasma membrane; the acrosome reaction (AR) is an exocytotic event induced by calcium influx, enabling the spermatozoa to penetrate the zona pellucida. These processes can be achieved only if the spermatozoa have good viability; indeed, determination of sperm viability is u...
متن کاملQuantitative analysis of flagellar movement in hyperactivated and acrosome-reacted golden hamster spermatozoa.
Caudal epididymal spermatozoa of golden hamsters were incubated in capacitation medium. Their movement patterns changed as they became hyperactivated and underwent the acrosome reaction. To understand the basic mechanism by which changes in movement pattern are brought about, digital image analysis was carried out on the flagellar movements recorded with a video system. The degree of flagellar ...
متن کاملAcrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs.
Many investigators maintain that spermatozoa that have initiated the acrosome reaction (AR) before reaching the surface of the egg's zona pellucida (ZP) are unable to bind and penetrate the ZP. A recent study has revealed that most fertilizing mouse spermatozoa initiate the AR before contacting the ZP. We found that acrosome-reacted spermatozoa collected from the perivitelline space of Cd9-null...
متن کاملTrypan blue exclusion assay by flow cytometry
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the...
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ژورنال
عنوان ژورنال: Nihon Chikusan Gakkaiho
سال: 1988
ISSN: 1346-907X,1880-8255
DOI: 10.2508/chikusan.59.235